Sandwich ELISA of human plasma using the recombinant human plasma antibody as the capture (100ng/well), and biotinylated anti-human light chains ( kappa;+ lambda;) antibody clone RM129 as the detect, followed by an alkaline phosphatase conjugated streptavidin.
Sandwich ELISA of human IgA1 using the recombinant Human IgA1 antibody as the capture (100ng/well), and biotinylated anti-human light chains ( kappa;+ lambda;) antibody clone RM129 as the detect, followed by an alkaline phosphatase conjugated streptavidin.
IHC testing of FFPE human tonsil tissue with recombinant Human IgA1 antibody. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.
ELISA of human immunoglobulins shows the recombinant Human IgA1 antibody reacts only to IgA. Very slightly cross reacts with IgA2. No cross reactivity with IgG, IgM, IgD, or IgE.
ELISA Titration: the plate was coated with different amounts of human IgA1. A serial dilution of recombinant Human IgA1 antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.
Supplier Page from NSJ Bioreagents for Human IgA1 Antibody [Recombinant Rabbit Monoclonal]